This issue is a variant of The Amazing Spider-Man (Marvel, 1999 series) #529. There exist further variants.
Part of a series of Spider-Man variant covers which feature the same motif of Spidey jumping at the viewer, but wearing a different costume each time.
"The Road to Civil War" tie-in.
House Advertisement for: ANNIHILATION: NOVA #1, ANNIHILATION: SILFER SURFER #1, ANNIHILATION: RONAN #1, ANNIHILATION: SUPER SKRULL #1.
Total of 10 one-page and 1 two-page advertisements for: Mirror Mask (dvd, Sony), NKE6.com (website, Nike), Spider-Man Plug & Play (toys, Jakks Pacific, 2 pages), Ultraviolet (movie, Sony), New York Comic-Con 2006 (1/2 page), Back Issues Online (comics, Midtown Comics, 1/2 page), The Hills Have Eyes (movie, Fox), The Joe Kubert School of Cartoon and Graphic Art (art school, Kubert), TNA Impact! (action figues, Marvel Toys), Fantastic Four: The Making of the Movie (book, Titan Books, 1/2 page), Comics Creators on Fantastic Four (book, Titan Books, 1/2 page), Abovetheinfluence.com (anti-drugs, Office of National Drug Control), Ultimate Avengers The Movie (dvd, Lion's Gate).