Batman [Bruce Wayne]; Batgirl [Oracle; Barbara Gordon]; Signal [Duke Thomas]; Batwoman [Kate Kane]; Spoiler [Stephanie Brown]; Orphan [Cassandra Cain]; Red Hood [Jason Todd]; Red Robin [Tim Drake]; Nightwing [Dick Grayson]; Clownhunter [Bao Pham]; Harvey Bullock; unnamed Gotham City police officers; The Joker; unnamed Joker henchmen; Alfred Pennyworth (animated corpse); Punchline [Alexis Kaye]; Underbroker [Harlan Graves]; Catwoman [Selina Kyle]; Harley Quinn [Harleen Quinzel]; Ghost-Maker (intro)
With Batman's allies fighting the Joker's henchmen throughout Gotham, and Nightwing facing off against Punchline, Batman battles the Joker to take back the city, while Harley Quinn plans to make sure it is the final confrontation between the two.
The Joker; Punchline [Alexis Kaye]; unnamed diner patron
A diner patron brings a mysterious stranger up to speed on current events in Gotham, including how Punchline, who is now in custody, is playing the role of a victim who claims that she was influenced by the Joker into participating in his crimes.
Story continues in Punchline (DC, 2021 series) #1.