The Fantastic Four [The Invisible Girl [Susan Storm]; The Human Torch [Johnny Storm]; The Thing [Ben Grimm]; Mr. Fantastic [Reed Richards]]; the Mole Man (villain)
The Mole Man arranges for the neighbors of the Fantastic Four to complain about their activities so they will be interested when they receive an ad for an island that is for sale. When they visit the island, the Mole Man is waiting for them.
Stan Lee (plot); Ernie Hart [H. E. Huntley] (script)
Ant-Man [Henry Pym] (origin detail); Wasp [Janet Van Dyne] (introduction, origin); Creature from Kosmos [Pilai] (introduction, named in a later comic); Maria Trovaya-Pym (flashback, introduction); Vernon Van Dyne (introduction); Lee Kearns (voice only)
When a fellow scientist is killed by an alien being, Ant-Man enlists the help of the dead man's daughter. He gives her the ability to shrink and tiny wasp wings, and she joins him as his partner the Wasp. The duo use the alien's acidic nature to destroy it, saving the city.